1998: T cells infiltrating coronary arteries of cardiac allografts with chronic rejection contain clonally expanded T lymphocytes Transplantation Proceedings 30(4): 1002-1004 2006: Tumor infiltrating lymphocytes in seminoma lesions comprise clonally expanded cytotoxic T cells International Journal of Cancer 119(4): 831-838 2023: Immune checkpoints are predominantly co-expressed by clonally expanded CD4+FoxP3+ intratumoral T-cells in primary human cancers Journal of Experimental and Clinical Cancer Research: Cr 42(1): 333 2024: Glioblastoma-infiltrating CD8+ T cells are predominantly a clonally expanded GZMK+ effector population Cancer Discovery 2024īredel, D. 2021: Circulating clonally expanded T cells reflect functions of tumor-infiltrating T cells Journal of Experimental Medicine 218(4) 2021: Tumor-Infiltrating B Lymphocyte Profiling Identifies IgG-Biased, Clonally Expanded Prognostic Phenotypes in Triple-Negative Breast Cancer Cancer Research 81(16): 4290-4304 2002: Distinct TCRAV and TCRBV repertoire and CDR3 sequence of T lymphocytes clonally expanded in blood and GVHD lesions after human allogeneic bone marrow transplantation Bone Marrow Transplantation 30(12): 915-923 2018: Human liver infiltrating γδ T cells are composed of clonally expanded circulating and tissue-resident populations Journal of hepatology 69(3): 654-665 2023: Immune phenotypes and checkpoint molecule expression of clonally expanded lymph node-infiltrating T cells in classical Hodgkin lymphoma Cancer Immunology Immunotherapy: Cii 72(2): 515-521 2019: Localization-associated immune phenotypes of clonally expanded tumor-infiltrating T cells and distribution of their target antigens in rectal cancer Oncoimmunology 8(6): E1586409īallhausen, A. 2021: Single-cell immune repertoire and transcriptome sequencing reveals that clonally expanded and transcriptionally distinct lymphocytes populate the aged central nervous system in mice PROCEEDINGS. 2021: Broadly reactive human CD4+ T cells against Enterobacteriaceae are found in the naïve repertoire and are clonally expanded in the memory repertoire European Journal of Immunology 51(3): 648-661 2015: Immune Repertoire Profiling Reveals that Clonally Expanded B and T Cells Infiltrating Diseased Human Kidneys Can Also Be Tracked in Blood Plos one 10(11): E0143125Ĭassotta, A. Our data suggest that expanded clonotypes in the tissues might be traceable in blood samples in the course of treatment or the natural history of the disease.

Immune repertoire analysis of tissue infiltrating B and T cells adds new approaches to the assessment of adaptive immune response in kidney diseases. In addition, complementarity determining region 3 sequences of the immunoglobulin heavy chains are on average more diverse than T cell receptor beta chains. Summarizing the data of all analyzed patients, 68% of highly expanded T cell clonotypes and 30% of the highly expanded B cell clonotypes that have infiltrated the kidney can be found amongst the five most abundant clonotypes in blood. While generally an individual's renal receptor repertoire is different from the repertoire present in blood, 94% (30/32) of the lymphocytes with clonal expansion in kidney can also be traced in blood however, not all of these clonotypes are equally abundant.

We used Biomed-2 primer panels and ImmunExplorer software to sequence, analyze and compare complementarity determining regions and V-(D)-J elements. In this study we compared Immunoglobulin and T cell receptor repertoires of lymphocytes found in kidney and blood samples of 10 patients with various renal diseases based on next-generation sequencing data. AbstractRecent advances in high-throughput sequencing allow for the competitive analysis of the human B and T cell immune repertoire.

Immune repertoire profiling